Cell culture is central to biomedical research, drug development, and the emerging cultivated meat industry. Yet, most in vitro systems poorly deliver oxygen, especially in 3D cultures. This results in hypoxic stress that alters cellular behaviour and reduces reproducibility.
We propose a novel insert for standard culture vessels that passively distributes oxygen using a biocompatible, oxygen-dissolving liquid. Unlike bubbling systems, this insert avoids shear damage and contamination risks. It’s re-usable, scalable, and adaptable to various formats.
The technology can improve organoid development, reduce animal testing, and standardise oxygenation in vitro. The insert also offers long-term sustainability advantages and can be applied in diverse sectors, including agriculture, where hypoxia is also a major issue.