One of the challenges in the healthcare is the introduction of molecular diagnostic tests which can improve diagnosis of the disease and choice of therapy in (cancer) patients. For this, it is important to prepare and transport frozen biomedical samples (example; tissues and other human materials) in a controlled and safe manner, so that the desired diagnostic tests can be performed in specialized laboratories. Due to increase in the number of molecular diagnostic tests, demand for a reliable cold-chain for preparation and transportation of bio samples is increasing rapidly. The cornerstone for sample preparation and logistics in the cryopreservation field is to use dry ice (solid carbon dioxide with a sublimation temperature of -78 °C). Unfortunately, dry ice cannot be directly used in freezing samples as the cooling rate, when a sample is gently place on a dry ice, is low. Therefore, clinical staff use hazardous substances such as isopentane that is pre-cooled by dry ice to freeze the sample. After freezing, the samples are transported in insulation boxes containing densified dry ice. A new technique is developed that not only cools the samples at a faster rate, but also eliminates the need of hazardous chemical prevalent in state-of-the-art workflows.